Smek1 Antibody

• Catalog Number: orb1246940
• Category: Antibodies
• Description: Smek1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EQQRERQDNPKLD
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SMEK1
• UniProt ID: Q6IN85
• NCBI: NP_115949.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SMEK homolog 1, suppressor of mek1 (Dictyostelium)
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 110kDa band observed in Human Pancreas lysates (calculated MW of 93.9kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246940 (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing SMEK1 and probed with orb1246940 (mock transfection in first lane).