SMEK1/KIAA2010 Antibody

• Catalog Number: orb19594
• Category: Antibodies
• Description: Goat polyclonal antibody to SMEK1
• Target: SMEK1 / KIAA2010
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EQQRERQDNPKLD
• RRID: AB_10749924
• MW: 93.9
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 110kDa band observed in Human Pancreas lysates (calculated MW of 93.9kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SMEK homolog 1, suppressor of mek1 (Dictyoste
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 55671

orb19594 (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK 293overexpressing SMEK1 (RC221040) and probed with orb19594 (mock transfection in first lane), tested by Origene.