You have no items in your shopping cart.
Cart summary
Item 1 of 8
Item 1 of 8
SMAD9 Antibody
Catalog Number: orb1254306
| Catalog Number | orb1254306 |
|---|---|
| Category | Antibodies |
| Description | SMAD9 Antibody |
| Target | SMAD9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMAD9 (NP_005896.1). |
| UniProt ID | O15198 |
| MW | Observed: 45-52kDa |
| Tested applications | IF, IHC, WB |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | SMAD9, SMAD, mothers against DPP homolog 9 (Drosop Read more... |
| Note | For research use only |
Western blot analysis of extracts of various cell lines, using SMAD9 antibody (orb1254306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver damage using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain cancer using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SMAD9 antibody (orb1254306). Blue: DAPI for nuclear staining.
- Item 1 of 4
- Item 1 of 4
SMAD9 Rabbit Polyclonal Antibody [orb6989]
FC, IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Equine, Gallus, Porcine
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 200 μl, 100 μl - Item 1 of 3
- Item 1 of 3
- Item 1 of 3
