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SMAD9 Antibody
SKU: orb1254306
Featured
Description
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMAD9 (NP_005896.1). |
| Target | SMAD9 |
| Molecular Weight | Observed: 45-52kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−SMAD9, SMAD, mothers against DPP homolog 9 (Drosophila)], HGNC:6774, MADH6, MADH9, SMAD8A, SMAD8B, MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, mothers against decapentaplegic homolog 9 (Drosophila), Mothers against decapentaplegic, drosophila, homolog of, 9
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Western blot analysis of extracts of various cell lines, using SMAD9 antibody (orb1254306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver damage using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain cancer using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SMAD9 antibody (orb1254306) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SMAD9 antibody (orb1254306). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
SMAD9
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
SMAD9 Antibody (orb1254306)
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