SMAD9 Rabbit Polyclonal Antibody

SKU: orb6989

Description

SMAD9 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Cell Signaling, Cytoplasmic, Hypoxia, Intracellular Signaling Proteins

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SMAD9 (361-467/467aa)
• Target: SMAD9
• Molecular Weight: 52 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

AI528653; BSP 1; BSP1; DKFZp781C1895; DKFZp781O1323; Dwarfin A; Dwf A; DwfA; Dwfc; hSMAD1; hSmad5; JV 41; JV4 1; JV41; JV5 1; MAD(mothers against decapentaplegic, Drosophila) homolog 1; MAD(mothers against decapentaplegic, Drosophila) homolog 9; MAD homolog 1; MAD homolog 9; MAD homolog1(mothers against decapentaplegic, Drosophila); Mad related protein 1; MAD, mothers against decapentaplegic homolog 9; MADH 1; Madh1; MADH5; MADH6; MADH9; Madr 1; MADR1; Mlp1; Mothers against decapentaplegic, drosophila, homolog of, 9; Mothers against DPP homolog 1; Mothers against DPP homolog 9; MusMLP; OTTHUMP00000178565; OTTMUSP00000022970; OTTMUSP00000022971; OTTMUSP00000022972; OTTMUSP0000002324; OTTMUSP00000023428; Sma and Mad related protein 1; Sma-and Mad-related protein 1; SMAD; SMAD family member 5; SMAD mothers against DPP homolog 1 Drosophila; SMAD8; SMAD8A; SMAD8B; Spinal muscular atrophy distal with upper limb predominance; TGF beta signaling protein 1; Transforming growth factor beta signaling protein 1; Transforming growth factor-beta signaling protein 1.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMAD9) Polyclonal Antibody, Unconjugated (orb6989) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Positive control: Hela cells, Concebtration: 3 µg/10^6 cells, Incubation conditions: Avoid light, 30 minutes on the ice.

Sample: Heart (Mouse) Lysate at 40 ug, Muscle (Mouse) Lysate at 40 ug, Primary: Anti-SMAD9 (orb6989) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Skin tissue lysates, Lane 3: Rat Testis tissue lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human A549 cell lysates, Lane 6: Human HepG2 cell lysates, Primary: Anti-SMAD9 (orb6989) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

SMAD9

UniProt

O15198