SMAD9 Rabbit Polyclonal Antibody

• Catalog Number: orb6989
• Category: Antibodies
• Description: SMAD9 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SMAD9 (361-467/467aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: SMAD9
• UniProt ID: O15198
• RRID: AB_10937886
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AI528653; BSP 1; BSP1; DKFZp781C1895; DKFZp781O132
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMAD9) Polyclonal Antibody, Unconjugated (orb6989) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Positive control: Hela cells, Concebtration: 3 µg/10^6 cells, Incubation conditions: Avoid light, 30 minutes on the ice.

Sample: Heart (Mouse) Lysate at 40 ug, Muscle (Mouse) Lysate at 40 ug, Primary: Anti-SMAD9 (orb6989) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Skin tissue lysates, Lane 3: Rat Testis tissue lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human A549 cell lysates, Lane 6: Human HepG2 cell lysates, Primary: Anti-SMAD9 (orb6989) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kDa, Observed band size: 50 kDa.