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Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; IF/ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 4G1C6 |
| Immunogen | Purified recombinant fragment of human SMAD4 expressed in E. Coli. |
| Target | Disease |
| Molecular Weight | 65kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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ELISA, IF, IHC, WB
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Polyclonal
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Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SMAD4 antibody

Western blot analysis of A431 (Lane1), SK-N-SH (Lane2), K562 (Lane3), HepG2 (Lane4) and HUVE12 (Lane5) cell lysate using SMAD4 antibody

Immunofluorescence analysis

Flow cytometry analysis of orb69305.
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SMAD4 Antibody (orb69305)
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