Smad4 Rabbit Polyclonal Antibody

• Catalog Number: orb526558
• Category: Antibodies
• Description: Smad4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Smad4 (451-552/552aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60 kDa
• Target: SMAD4
• UniProt ID: Q13485
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Mothers against decapentaplegic homolog 4; (Small)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Mousse Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Smad4 Polyclonal Antibody, Unconjugated (orb526558) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Lane 1: Human K562 cell lysates, Lane 2: Human Hela cell lysates, Lane 3: Human Jurkat cell lysates, Lane 4: Human HL-60 Cell Lysates, Primary: Anti-Smad4 (orb526558) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 60 kDa.

Sample: Siha (Human) Cell Lysate at 30 ug, HL-60 (Human) Cell Lysate at 30 ug, Primary: Anti-Smad4 (orb526558) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 63 kD.