Smad4 Rabbit Polyclonal Antibody

SKU: orb526558

Description

Smad4 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: WB
• Dilution range: WB=1:500-2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat, Sheep, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Smad4 (451-552/552aa)
• Target: SMAD4
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Mothers against decapentaplegic homolog 4; (Small) Mothers Against Decapentaplegic; Deleted in Pancreatic Carcinoma; Deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; DPC 4; DPC4; hSMAD4; JIP; MAD homolog 4; MAD mothers against decapentaplegic Drosophila homolog 4; MAD mothers against decapentaplegic homolog 4; MADH 4; MADH4; Med; Medea; Mothers against decapentaplegic homolog 4; Mothers against DPP homolog 4; OTTHUMP00000163548; Smad 4; SMAD family member 4; SMAD mothers against DPP homolog 4.

Paraformaldehyde-fixed, paraffin embedded Mousse Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Smad4 Polyclonal Antibody, Unconjugated (orb526558) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Lane 1: Human K562 cell lysates, Lane 2: Human Hela cell lysates, Lane 3: Human Jurkat cell lysates, Lane 4: Human HL-60 Cell Lysates, Primary: Anti-Smad4 (orb526558) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 60 kDa.

Sample: Siha (Human) Cell Lysate at 30 ug, HL-60 (Human) Cell Lysate at 30 ug, Primary: Anti-Smad4 (orb526558) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 63 kD.

Quick Database Links

Gene Symbol

SMAD4

UniProt

Q13485