SLC7A11 Antibody

SKU: orb1247762

Description

SLC7A11 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50-55kDa band observed in lysates of cell lines A549 and U2OS, and in Human Smooth Muscle and Tonsil lysates (calculated MW of 55.4kDa according to NP_055146.1). Recommended concentration: 0.03-0.3µg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 3.75µg/mL.Immunofluorescence: Strong expression of the protein seen in the vesicles/ cytoplasm of A549 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KGQTQNFKDAFSGRD
• Target: SLC7A11
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SLC7A11, solute carrier family 7, (cationic amino acid transporter, y+ system) member 11, CCBR1, xCT , cystine/glutamate transporter

orb1247762 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247762 (3.75 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247762 (0.3 ug/ml) staining of A549 (A) and U2OS (B) cell ysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247762 (0.1 ug/ml) staining of Human Smooth Muscle (A) and Tonsil (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247762 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing vesicle/cytoplasmic staining.

orb1247762 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

SLC7A11

UniProt

Q9UPY5

RefSeq (Protein):NP_055146.1