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SLC6A4 Antibody

SKU: orb1248752

Description

SLC6A4 Antibody

Images & Validation

Tested ApplicationsELISA, FC, IF
ReactivityHuman
Predicted ReactivityBovine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus/vesicles of HepG2 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-KERIIKSITPETPT
TargetSLC6A4
Molecular WeightApprox 75 kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SLC6A4, solute carrier family 6 (neurotransmitter transporter, serotonin), member 4, 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, hSERT, 5-hydroxytryptamine transporter, 5HT transporter, Na+/Cl- dependent serotonin transporter, OTTHUMP00000163632, OTTHUMP00000163633, sodium-dependent serotonin transporter, solute carrier family 6 member 4

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SLC6A4 Antibody

orb1248752 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi apparatus/vesicle staining.

SLC6A4 Antibody

orb1248752 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

SLC6A4 Antibody (orb1248752)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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