Anti-SERT [8B6]

• Catalog Number: orb1152488
• Category: Antibodies
• Description: Mouse monoclonal antibody to SERT
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8B6
• Tested applications: Crystallography
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: 8B6 was produced by immunising SERT KO mice with residues 73-616 of wildtype human SERT. Hybridomas were generated using established protocols.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: SERT
• UniProt ID: P31645
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: SLC6A4; Sodium-dependent serotonin transporter; 5H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-SERT antibody 8B6. Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 8B6 (orb1152487, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HepG2 cells with anti-SERT 8B6. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric rabbit IgG version of 8B6 (orb1152487) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152487, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.