SLC6A4 Antibody

• Catalog Number: orb1246948
• Category: Antibodies
• Description: SLC6A4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-LHQGERETWGK
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SLC6A4
• UniProt ID: P31645
• NCBI: NP_001036.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SLC6A4, SERT, solute carrier family 6 (neurotransm
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70-75kDa band observed in Human Colon lysates (calculated MW of 70.3kDa according to NP_001036.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in vesicles of HepG2 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246948 (2 ug/ml) staining of Human Colon lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246948 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).