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Catalog Number | orb1246948 |
---|---|
Category | Antibodies |
Description | SLC6A4 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-LHQGERETWGK |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70-75kDa band observed in Human Colon lysates (calculated MW of 70.3kDa according to NP_001036.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in vesicles of HepG2 cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | SLC6A4 |
UniProt ID | P31645 |
NCBI | NP_001036.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | SLC6A4, SERT, solute carrier family 6 (neurotransm Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70-75kDa band observed in Human Colon lysates (calculated MW of 70.3kDa according to NP_001036.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in vesicles of HepG2 cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246948 (2 ug/ml) staining of Human Colon lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1246948 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).
ELISA, IHC | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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