SIRT3 Antibody, KO Validated

• Catalog Number: orb1274444
• Category: Antibodies
• Description: SIRT3 Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 28kDa
• Target: SIRT3
• UniProt ID: Q9NTG7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: SIRT3 Antibody: SIR2L3, SIR2L3, NAD-dependent prot
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of SW620 cells, using SIRT3 antibody (orb1274444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) HeLa cells, using SIRT3 antibody (orb1274444) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (orb1274444) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using SIRT3 antibody (orb1274444) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using SIRT3 antibody (orb1274444) at dilution of 1:100 (40x lens).