IL2RA Antibody

SKU: orb1272673

Description

IL2RA Antibody

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Human
• Application Notes:
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Rα (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required, in the presence of 0.5 ng/mL recombinant hIL-2. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human sIL-2Rα when used in conjunction with compatible secondary reagents. Western Blot:To detect hsIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Rα is 2.0-4.0 ng/lane, under reducing or non-reducing conditions

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: SB-647
• Immunogen: sIL-2Ra antibody is produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen.
• Target: IL2RA
• Purification: This IgG2aK antibody was purified from cell culture by Protein G affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-2 receptor subunit alpha, TAC antigen, IL-2 receptor subunit alpha, CD25, IL2R, TCGFR, IDDM10

This antibody stained frozen human tonsil. The recommended concentration is 5.0 ug/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

IL2RA

UniProt

P01589

RefSeq:P01589