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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 6
| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; IF/ICC: 1/200 - 1/1000; ELISA: 1/10000 |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 1B5D12 |
| Immunogen | Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli. |
| Target | Disease |
| Molecular Weight | 30.8kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD25; IL2R; TCGFR; IDDM10
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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of IL2RA antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rectum cancer tissues using IL2RA antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded bladder cancer tissues using IL2RA antibody
Western blot analysis of human IL2RA using IL2RA antibody
Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using IL2RA antibody
Western blot analysis of Hela (Lane 1), MOLT4 (Lane 2), HEK293 (Lane 3), A549 (Lane 4), Jurkat (Lane 5), K562 (Lane 6), Cos7 (Lane 7), PC-12 (Lane 8) NIH/3T3 (Lane 9) cell lysate using IL2RA antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IL2RA Antibody (orb153477)
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