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SHP2 Antibody (Y546)

Catalog Number: orb1928476

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1928476
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB21493
Tested applicationsFC, IHC-P, WB
Predicted ReactivityMouse, Rat
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeWB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW68011 Da
TargetThis SHP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 526-551 amino acids from human SHP2.
UniProt IDQ06124
NCBINP_542168.1, NP_002825.3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesTyrosine-protein phosphatase non-receptor type 11,
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NoteFor research use only
Expiration Date12 months from date of receipt.
SHP2 Antibody (Y546)

Western blot analysis of SHP2 Antibody (Y546) in 293, K562 cell line lysates (35 ug/lane). SHP2 (arrow) was detected using the purified Pab.

SHP2 Antibody (Y546)

Flow cytometric analysis of 293 cells using SHP2 Antibody (Y546) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SHP2 Antibody (Y546)

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SHP2 Antibody (Y546), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.