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Item 1 of 3
Phospho-SHP2(Y546) Antibody
Catalog Number: orb1788334
| Catalog Number | orb1788334 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Immunogen | 521-551 aa |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, DB: 1:500 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 68011 Da |
| Target | This SHP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y546 of human SHP2. |
| UniProt ID | Q06124 |
| NCBI | NP_002825.3, NP_542168.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | PTPN11; PTP2C; SHPTP2; Tyrosine-protein phosphatas Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-SHP2-Y546 Antibody (EV) (upper) or Beta-actin (lower).
Dot blot analysis of anti-Phospho-SHP2-Y546 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Western blot analysis of lysate from NIH/3T3 cell line, using Phospho-SHP2-Y546 Antibody. Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
