SH3GL1 Antibody

• Catalog Number: orb1249257
• Category: Antibodies
• Description: SH3GL1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EPFDLGEPEQSN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in lysates of cell line K562 (calculated MW of 41.5kDa according to NP_003016.1. The observed molecular weight corresponds to earlier findings in literature with different antibodies (So et al, Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2563-8.; PMID: 9122235). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SH3GL1
• UniProt ID: Q99961
• NCBI: NP_003016.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SH3GL1, SH3-domain GRB2-like 1, CNSA1, EEN, MGC111
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in lysates of cell line K562 (calculated MW of 41.5kDa according to NP_003016.1. The observed molecular weight corresponds to earlier findings in literature with different antibodies (So et al, Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2563-8.; PMID: 9122235). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249257 (0.3 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249257 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.