Endophilin-A2 Antibody

• Catalog Number: orb22522
• Category: Antibodies
• Description: Goat polyclonal antibody to SH3GL1
• Target: Endophilin-A2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EPFDLGEPEQSN
• RRID: AB_10930962
• MW: 41.5
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 48kDa band observed in lysates of cell line K562 (calculated MW of 41.5kDa according to NP_003016.1. The observed molecular weight corresponds to earlier findings in literature with different antibodies (So et al, Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2563-8.; PMID: 9122235). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SH3GL1 antibody, anti SH3-domain GRB2-like 1
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Entrez: 6455

Western blot analysis of K562 lysate using SH3GL1 antibody