SH3BP1 Antibody

• Catalog Number: orb1246956
• Category: Antibodies
• Description: SH3BP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-PQPRPRSLASETN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7kDa according to NP_061830.3). Recommended for use at 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3ug/ml.Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SH3BP1
• UniProt ID: Q9Y3L3
• NCBI: NP_061830.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SH3BP1, SH3-domain binding protein 1, SH3 domain b
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7kDa according to NP_061830.3). Recommended for use at 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3ug/ml.Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).
• Expiration Date: 12 months from date of receipt.

orb1246956 staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1246956 (0.3 ug/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.