Goat anti-SH3BP1 Antibody

• Catalog Number: orb18633
• Category: Antibodies
• Description: Goat polyclonal antibody to SH3BP1
• Target: SH3BP1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PQPRPRSLASETN
• RRID: AB_10752522
• MW: 75.7
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration, 0.3ug/ml.WB: Approx 70kDa band observed in Human Lung and Kidney lysates (predicted MW of 71kDa according to NP_061830). Recommended for use at 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SH3BP1 antibody, anti SH3-domain binding prot
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 23616

orb18633 staining (1ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18633 (0.3ug/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.