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Catalog Number | orb18633 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to SH3BP1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, IHC, WB |
Reactivity | Human |
Dilution range | ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 0.3 μg/ml |
Conjugation | Unconjugated |
MW | 75.7 |
Target | SH3BP1 |
Entrez | 23616 |
Protein Sequence | PQPRPRSLASETN |
RRID | AB_10752522 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti SH3BP1 antibody, anti SH3-domain binding prot Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration, 0.3ug/ml.WB: Approx 70kDa band observed in Human Lung and Kidney lysates (predicted MW of 71kDa according to NP_061830). Recommended for use at 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb18633 staining (1ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
orb18633 (0.3ug/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.