SH2D3A / NSP1 Antibody

• Catalog Number: orb1248611
• Category: Antibodies
• Description: SH2D3A / NSP1 Antibody
• Target: SH2D3A / NSP1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-APRAERFEKFQR
• UniProt ID: Q9BRG2
• MW: Approx 60kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 60kDa band observed in Human Lung and Tonsil lysates (calculated MW of 63.1kDa according to NP_005481.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SH2D3A, NSP1, SH2 domain containing 3A, SH2 domain
• Research Area: Metabolism Research, Neuroscience
• Note: For research use only
• NCBI: NP_005481.2

orb1248611 (0.1 ug/ml) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248611 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.