Goat anti-SH2D3A / NSP1 Antibody

• Catalog Number: orb20171
• Category: Antibodies
• Description: Goat polyclonal antibody to SH2D3A
• Target: SH2D3A / NSP1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: APRAERFEKFQR
• RRID: AB_10748573
• MW: 63.1
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 60kDa band observed in Human Lung and Tonsil lysates (calculated MW of 63.1kDa according to NP_005481.2). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SH2D3A antibody, anti NSP1 antibody, anti SH2
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 10045

orb20171 (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.