SET Antibody

SKU: orb1260886

Description

SET Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 146-277 of human SET (NP_003002.2).
• Target: SET
• Molecular Weight: Observed: 34kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

SET, SET translocation (myeloid leukemia-associated), 2PP2A, I2PP2A, PHAPII, TAF-IBETA, SET, translocation, IGAAD, inhibitor-2 of protein phosphatase-2A, Inhibitor of GZMA-activated DNAse, TAF-I, IPP2A2

Western blot analysis of extracts of various cell lines, using SET antibody (orb1260886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat liver using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug BAX antibody (orb1274527). Western blot was performed from the immunoprecipitate using BAX antibody (orb1274527) at a dilution of 1:1000.

Immunohistochemistry of paraffin-embedded mouse kidney using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using SET antibody (orb1260886) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

SET

UniProt

Q01105