SERCA2/ATP2A2 Antibody

SKU: orb1248312

Description

SERCA2/ATP2A2 Antibody

Research Area

Cancer Biology, Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110kDa according to NP_001672.1). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: DELNPSAQRDACLN
• Target: SERCA2 / ATP2A2
• Molecular Weight: Approx 110kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP2A2, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2, ATP2B, DAR, DD, DKFZp686P0211, FLJ20293, FLJ38063, MGC45367, SERCA2, ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2, SR Ca(2+)-ATPase 2, calcium pump 2, calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, cardiac Ca2+ ATPase, endoplasmic reticulum class 1/2 Ca(2+) ATPase, sarcoplasmic/endoplasmic reticulum calcium ATPase 2

orb1248312 (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248312 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

SERCA2 / ATP2A2

UniProt

P16615

RefSeq (Protein):NP_733765.1, NP_001672.1