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Item 1 of 4
Item 1 of 4
Goat anti-SERCA2 / ATP2A2 Antibody
Catalog Number: orb153642
| Catalog Number | orb153642 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal to SERCA2. |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC, WB |
| Reactivity | Canine, Human, Mouse, Porcine, Rat |
| Dilution range | ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml |
| Conjugation | Unconjugated |
| MW | 110; 115 |
| Target | SERCA2 / ATP2A2 |
| Entrez | 488 |
| Protein Sequence | DELNPSAQRDACLN |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti ATP2B antibody, anti ATPase, Ca++ dependent, Read more... |
| Note | For research use only |
| Application notes | WB: Approx 110 kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110 kDa according to NP_001672.1). Recommended concentration: 0.1-0.3 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
0.1 μg/ml staining of A549 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.
5 μg/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
mmunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
0.1 μg/ml staining of Human Heart (A), Mouse Heart (B), Rat Heart (C) and Mouse Smooth Muscle (D) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.
