SERCA2/ATP2A2 Antibody

SKU: orb153642

Description

Goat polyclonal to SERCA2.

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  WB: Approx 110 kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110 kDa according to NP_001672.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SERCA2 / ATP2A2
• Molecular Weight: 110; 115
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti ATP2B antibody, anti ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2 antibody, anti ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 antibody, anti calcium pump 2 antibody, anti calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform antibody, anti cardiac Ca2+ ATPase antibody, anti DAR antibody, anti DD antibody, anti DKFZp686P0211 antibody, anti endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody, anti FLJ20293 antibody, anti FLJ38063 antibody, anti MGC45367 antibody, anti sarcoplasmic/endoplasmic reticulum calcium ATPase 2 antibody, anti SERCA2 antibody, anti SR Ca(2+)-ATPase 2 antibody, anti ATP2A2 antibody

0.1 μg/ml staining of A549 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

5 μg/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

mmunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

0.1 μg/ml staining of Human Heart (A), Mouse Heart (B), Rat Heart (C) and Mouse Smooth Muscle (D) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

SERCA2 / ATP2A2

NCBI Gene ID

488