SELE Antibody

• Catalog Number: orb1258228
• Category: Antibodies
• Description: SELE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human SELE (NP_000441.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: SELE
• UniProt ID: P16581
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SELE, selectin E, CD62E, ELAM, ELAM1, ESEL, LECAM2
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SELE antibody (orb1258228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human breast using SELE Antibody (orb1258228) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterus using SELE Antibody (orb1258228) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using SELE Antibody (orb1258228) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF-7 cells using SELE antibody (orb1258228). Blue: DAPI for nuclear staining.