E-Selectin Rabbit Polyclonal Antibody

SKU: orb10342

Description

E-Selectin Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse E-Selectin (501-612/612aa)
• Target: SELE
• Molecular Weight: 115 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD62E; ELAM; ELAM1; ESEL; LECAM2; selectin-e; E-selectin; ELAM-1; LYAM2_HUMAN; SELE; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1 (ELAM-1); Leukocyte-endothelial cell adhesion molecule 2 (LECAM2); LYAM2_MOUSE; LYAM2_RAT;

100 ng rhE-Selectin-His protein per lane probed with E-Selectin polyclonal antibody respectively, unconjugated (orb10342) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with E-Selectin Polyclonal Antibody, Unconjugated (orb10342) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

SELE

UniProt

Q00690