E-Selectin Rabbit Polyclonal Antibody

• Catalog Number: orb10342
• Category: Antibodies
• Description: E-Selectin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse E-Selectin (501-612/612aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 115 kDa
• Target: SELE
• UniProt ID: Q00690
• RRID: AB_10750697
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD 62E; CD26E; CD62E antigen; CD62 antigen-like fa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

100 ng rhE-Selectin-His protein per lane probed with E-Selectin polyclonal antibody respectively, unconjugated (orb10342) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with E-Selectin Polyclonal Antibody, Unconjugated (orb10342) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.