SCD Rabbit Polyclonal Antibody

SKU: orb704252

Description

SCD Rabbit Polyclonal Antibody

Research Area

Cell Biology, Fatty Acid Oxidation, Fatty Acids, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism, Obesity

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SCD (200-359/359aa)
• Target: SCD
• Molecular Weight: 41 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

SCD_HUMAN; Stearoyl-CoA desaturase; EC:1.14.19.1; hSCD1; Acyl-CoA desaturase; delta(9)-desaturase(delta-9 desaturase); Fatty acid desaturase; FADS5; SCD1; SCDOS; MSTP008;

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SCD) Polyclonal Antibody, Unconjugated (orb704252) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SCD) Polyclonal Antibody, Unconjugated (orb704252) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Hela (Human) Cell Lysate at 25 ug, Lane 2: SCD knockout (KO) Hela (Human) Cell Lysate at 25 ug, Primary: Anti-RHOC (orb704252) at 1/1000 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 38 kD, Observed band size: 38 kD.

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-SCD (orb704252) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38 kD, Observed band size: 38 kD.

U2-OS cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (KO Validated) SCD polyclonal Antibody, Unconjugated (orb704252) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

SCD

UniProt

O00767

References

C Zalambani, L Anconelli, N Calonghi, D Telese, G Micheletti, C Boga, G Farruggia… New Compounds with Enhanced Biological Activity Through the Strategic Introduction of Silylated Groups into Hydroxystearic Acids Molecules,, (2025)

PubMed:

Applications

WB

Reactivity

Human