SARS-CoV-2 Spike P681H Antibody (Alpha, Mu Variant)

• Catalog Number: orb1238673
• Category: Tools
• Description: SARS-CoV-2 Spike P681H Antibody (Alpha, Mu Variant)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike P681H Antibody (orb1238673) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 UK variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 UK variant (B.1.1.7) Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL.Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody can specifically detect SARS-CoV-2 UK Variant (B.1.1.7) Spike S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA. It can also detect mutant peptide (681H), but not WT peptide (681P). All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2°COVID-19) P681H Mutant Specific Spike antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 µg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 UK Variant Spike antibody, orb1238673, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238673) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (orb1231589).

WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7). Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1238673, 1 µg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238673) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (orb1231589).

ELISA Validation of P681H Mutant Specific Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and UK variant (B.1.1.7), 1 µg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1239981, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. Immunogen region of antibody orb1239981 includes site 681P that was mutated in B.1.1.7. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238673) can specifically detect UK variant spike S1 peptide (681H), but not WT peptide (681P).