SARS-CoV-2 Spike P681H Antibody [1G8D11] (Alpha, Mu Variant)

• Catalog Number: orb1238731
• Category: Antibodies
• Description: SARS-CoV-2 Spike P681H Antibody [1G8D11] (Alpha, Mu Variant)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1G8D11
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG2a, Kappa
• Immunogen: Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike P681H antibody (orb1238731) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Alpha variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 Alpha Variant (B.1.1.7, UK) Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL.Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody can specifically detect SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA and WB. It can also detect mutant peptide (681H), but not WT peptide (681P). All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2°COVID-19) P681H Mutant Specific Spike antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants.Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, orb1238731, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr. SARS-CoV-2 alpha variant spike antibody (orb1238731) can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants by ELISA.

ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein.Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, orb1238731, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238731) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (orb1231589) by ELISA.

WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein.Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1238731, 1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238731) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (orb1231589) by WB.

ELISA Validation of P681H Mutant Specific Spike Antibodies with Mutant and WT Peptide.Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT (orb1231701) and alpha variant (B.1.1.7, UK) (orb1231664), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1238731, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238731) can specifically detect alpha variant spike S1 peptide (681H, orb1231664), but not WT peptide (681P, orb1231701).