SARS-CoV-2 Spike P26S Antibody (Gamma Variant)

• Catalog Number: orb1239024
• Category: Antibodies
• Description: SARS-CoV-2 Spike P26S Antibody (Gamma Variant)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody (orb1239024) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Gamma variant (P.1) Spike protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 Gamma variant (P.1) Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL.Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2 Spike P26S antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 Spike P26S antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 µg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, orb1239024, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr.

ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 µg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, orb1239024, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody (orb1239024) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (orb1231589).

WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1). Detection Antibodies: Spike P26S (Gamma Variant) antibody, orb1239024, 1 µg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody (orb1239024) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (orb1231589).