SARS-CoV-2 (COVID-19) Spike P26S Antibody [5G12G11] (Gamma Variant)

• Catalog Number: orb1238709
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike P26S Antibody [5G12G11] (Gamma Variant)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5G12G11
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG1
• Immunogen: Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody (orb1238709) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Gamma variant (P.1) Spike protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 Gamma variant (P.1) Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL.Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2 Spike P26S (Gamma Variant) antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 Spike P26S (Gamma Variant) antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 Spike 156-157EF antibody: Severe acute
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr.

ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein.Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, orb1238709, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (orb1231589).

WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein.Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1). Detection Antibodies: Spike P26S (Gamma Variant) antibody, orb1238709, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (orb1231589).

ELISA Validation of Gamma Variant Spike S1 Antibodies with Mutant and WT Peptide.Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (orb1231703) and Gamma variant (P.1) (orb1231659), 1 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, orb1238709, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody detects Gamma variant spike S1 peptide (26S, orb1231659), but not WT peptide (26P, orb1231703).