SARS-CoV-2 NP (84C4a) rabbit mAb

• Catalog Number: orb1945990
• Category: Antibodies
• Description: SARS-CoV-2 NP (84C4a) rabbit mAb
• Clonality: Monoclonal
• Clone Number: CoV2NP-84C4a
• Tested applications: ELISA
• Reactivity: Other
• Isotype: Rabbit IgGk
• Immunogen: SARS-CoV-2 nucleoprotein (NP) specific peptide
• Concentration: 0.5 mg/ml
• Dilution range: Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
• Conjugation: Unconjugated
• UniProt ID: P0DTC9
• Storage: 2-8°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3
• Alternative names: Severe acute respiratory syndrome coronavirus 2 nu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal antibody (84C4a), CoV2NP-84C4. Cat. #orb1945990.

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 84C4a (Cat# orb1945990) was serially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a (Cat# orb1945990) as a capture antibody and 75G5a-biotin (Cat# orb1945991) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).

Affinity measurement of SARS-CoV-2 (84C4a) antibody.