SARS-CoV-2 NP (75G5a) rabbit mAb Biotin Conjugate Antibody

• Catalog Number: orb1945991
• Category: Antibodies
• Description: SARS-CoV-2 NP (75G5a) rabbit mAb Biotin Conjugate Antibody
• Clonality: Monoclonal
• Clone Number: CoV2NP-75G5a
• Tested applications: ELISA
• Reactivity: Other
• Isotype: Rabbit IgGk
• Immunogen: SARS-CoV-2 nucleoprotein (NP) specific peptide
• Concentration: 0.5 mg/ml
• Dilution range: Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
• Conjugation: Biotin
• UniProt ID: P0DTC9
• Storage: 2-8°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3
• Alternative names: Severe acute respiratory syndrome coronavirus 2 nu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) at 1 ug/mL. Biotin-conjugated rabbit monoclonal antibody 75G5a (Cat# orb1945991) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.

Western blot analysis of SARS-CoV-2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP nucleocapsid rabbit monoclonal antibody (75G5), CoV2NP-75G5a), Cat# orb1945992.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a (Cat# orb1945990) as a capture antibody and 75G5a-biotin (Cat# orb1945991) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 85C1 (Cat# orb1945986) as a capture antibody and 75G5a-biotin (Cat# orb1945991) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 85C1 and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 25 pg/mL (2.5 pg).