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Catalog Number | orb1231607 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein |
Tested applications | ELISA, WB |
Tag | mFc tag at the C-terminus |
Dilution range | This recombinant protein can be used for SDS-PAGE. |
Form/Appearance | Lyophilized |
Purity | > 95% by SDS-PAGE. |
MW | 50.8 kDa |
Target | S |
UniProt ID | P0DTC2 |
Protein Sequence | Arg319-Phe541 |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV RBD-mFc at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 8-80 ng/mL. |
NCBI | YP_009724390.1 |
Storage | Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | S1-RBD protein, NCP-CoV RBD Protein, novel coronav Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for SDS-PAGE. |
Expiration Date | 6 months from date of receipt. |
Immobilized SARS-CoV-2 (COVID-19) spike RBD recombinant protein at 2μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 8-80 ng/mL.
SARS-CoV-2 (COVID-19) spike RBD recombinant protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 60 kD.
ELISA, SDS-PAGE, WB | |
> 95% as determined by Bis-Tris PAGE | |
35 kD | |
HEK293 cells |
ELISA, SDS-PAGE, WB | |
> 90% as determined by Bis-Tris PAGE | |
120 kD | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLCEndotoxin level is less than 1EU per ug by the LAL method. | |
HEK293 cells |
ELISA, WB | |
< 95% | |
42.9 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
31.8 kD | |
HEK293 cells |
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