SARS-CoV-2 (COVID-19) Spike Antibody (biotin)

• Catalog Number: orb1239045
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike Antibody (biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike antibody (3525) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
• Concentration: 1 mg/mL
• Dilution range: IHC: 1 μg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Biotin
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2°COVID-19) Spike antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute
• Note: For research use only
• Application notes: IHC: 1 μg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) spike antibody (orb1239045, 2 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C, following by streptavidin-HRP conjugate at 20 ug/mL. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation. Coating Antigen: immunogen peptide, orb1236596, 10 ug/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1239045 or orb1239976, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1239045 was detected by HRP-conjugated streptavidin at 1:5000 and orb1239976 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10000, incubate at RT for 1 hr.