SARS-CoV-2 (COVID-19) Spike ECD Monoclonal Antibody [B004] (Biotin)

• Catalog Number: orb1273999
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike ECD Monoclonal Antibody [B004] (Biotin)
• Species/Host: Human
• Clonality: Monoclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Virus
• Immunogen: S-ECD recombinant protein
• Concentration: batch dependent
• Dilution range: WB: 1:1000~2000
• Form/Appearance: Liquid
• Conjugation: Biotin
• Target: S
• NCBI: QHD43416.1
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 10% glycerol, pH 7.2
• Alternative names: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody: S
• Note: For research use only
• Application notes: WB: 1:1000~2000
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with Recombinant Protein. Loading: 1 ug of recombinant protein per lane. Antibodies: ARS-CoV-2 (COVID-19, 2019-nCoV) Spike-ECD Monoclonal, orb1273999, 1:500. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.

ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein, orb1231624, and column 2: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein. Antibodies: SARS-CoV-2 (COVID-19) Spike-ECD Monoclonal Antibody, orb1273999. Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution. Develop: 15 min, 100 uL/well. Stop: Stop buffer 50 uL/well.