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Catalog Number | orb1231468 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (COVID-19) S2 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His Tag |
Form/Appearance | Liquid |
Purity | > 95% as determined by SDS-PAGE. |
MW | 60.0 kDa |
Target | S |
UniProt ID | P0DTC2 |
Source | HEK293 cells |
NCBI | QHD43416.1 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 20 mM PB, 300 mM NaCl, pH 7.4 |
Alternative names | Spike,S2 protein,Spike glycoprotein Subunit2,S gly Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.0 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SARS-CoV-2 S2 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized SARS-CoV-2 S2 protein, His Tag at 1 ug/ml (100 uL/well) can bind SARS-CoV-2 Spike S2 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL.
ELISA, WB | |
< 95% | |
42.9 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
31.8 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
24.7 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
76.2 kD | |
HEK293 cells |
WB | |
> 95% by SDS-PAGE. | |
HEK293 cells |