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Catalog Number | orb1231444 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (COVID-19) S Recombinant Protein RBD |
Tested applications | ELISA, WB |
Tag | His Tag |
Dilution range | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Form/Appearance | Liquid |
Purity | > 95% as determined by SDS-PAGE. |
MW | 27.0 kDa |
Target | S |
UniProt ID | P0DTC2 |
Source | HEK293 cells |
NCBI | QHD43416.1 |
Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 10 mM PB, pH 7.4 |
Alternative names | Spike,S protein RBD,Spike glycoprotein Receptor-bi Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SARS-CoV-2 S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized SARS-CoV-2 S protein RBD, His Tag at 1 ug/ml (100 uL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL.
ELISA, SDS-PAGE, WB | |
> 95% as determined by Bis-Tris PAGE | |
35 kD | |
HEK293 cells |
ELISA, SDS-PAGE, WB | |
> 90% as determined by Bis-Tris PAGE | |
120 kD | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLCEndotoxin level is less than 1EU per ug by the LAL method. | |
HEK293 cells |
ELISA, WB | |
< 95% | |
42.9 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
31.8 kD | |
HEK293 cells |
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