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Catalog Number | orb1231620 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (COVID-19) RBD Recombinant Protein |
Tested applications | ELISA |
Concentration | 0.5 mg/ml |
Dilution range | ELISA |
Form/Appearance | Liquid |
Purity | > 95% by SDS Page |
MW | The predicted molecular mass is ~33 kDa. |
Protein Sequence | RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFK CYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWN SNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGF QPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESN KKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCS |
Source | HEK293 cells |
NCBI | QHD43416.1 |
Storage | This recombinant protein may be stored as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Buffer/Preservatives | This recombinant protein is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added. |
Note | For research use only |
Application notes | ELISA |
Expiration Date | 6 months from date of receipt. |
Purified SARS-CoV-2 Receptor Binding Domain (RBD) Spike Protein under non-reducing conditions. Lane 1-RBD Protein loaded at 10 μg. Lane 2-RBD Protein loaded 20 μg
ELISA, SDS-PAGE, WB | |
> 95% as determined by Bis-Tris PAGE | |
35 kD | |
HEK293 cells |
ELISA, SDS-PAGE, WB | |
> 90% as determined by Bis-Tris PAGE | |
120 kD | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLCEndotoxin level is less than 1EU per ug by the LAL method. | |
HEK293 cells |
ELISA, WB | |
< 95% | |
42.9 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
31.8 kD | |
HEK293 cells |
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