SAR1B Antibody

SKU: orb1246984

Description

SAR1B Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 25kDa band observed in Mouse Liver and Rat Liver lysates (calculated MW of 22.4kDa according to human NP_057187.1 and mouse NP_079811.1). In transfected HEK293 transiently expressing SAR1B a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DHERLLESKEELD
• Target: SAR1B
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SAR1B, SARA2, SAR1 gene homolog B (S. cerevisiae), CMRD, GTBPB, 2310075M17Rik, GTP-binding protein Sara, SAR1a gene homolog 2

orb1246984 (0.03 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing SAR1B and probed with orb1246984 (mock transfection in first lane).

Quick Database Links

Gene Symbol

SAR1B

UniProt

Q9Y6B6

RefSeq (Protein):NP_001028675.1, NP_057187.1