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SAR1B/SARA2 Antibody

SKU: orb19583

Description

Goat polyclonal antibody to SAR1B

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:8000, WB: 0.03-0.1 μg/ml
ReactivityMouse, Rat
Predicted ReactivityHuman
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 25kDa band observed in Mouse Liver and Rat Liver lysates (calculated MW of 22.4kDa according to human NP_057187.1 and mouse NP_079811.1). In transfected HEK293 transiently expressing SAR1B a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

ClonalityPolyclonal
TargetSAR1B / SARA2
Protein SequenceDHERLLESKEELD
Molecular Weight22.4, 22.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti SAR1B antibody, anti SARA2 antibody, anti SAR1 gene homolog B (S. cerevisiae) antibody, anti CMRD antibody, anti GTBPB antibody, anti 2310075M17Rik antibody, anti GTP-binding protein Sara antibody, anti SAR1a gene homolog 2 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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