SAR1B/SARA2 Antibody

• Catalog Number: orb19583
• Category: Antibodies
• Description: Goat polyclonal antibody to SAR1B
• Target: SAR1B / SARA2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DHERLLESKEELD
• RRID: AB_10751434
• MW: 22.4, 22.4
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.03-0.1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 25kDa band observed in Mouse Liver and Rat Liver lysates (calculated MW of 22.4kDa according to human NP_057187.1 and mouse NP_079811.1). In transfected HEK293 transiently expressing SAR1B a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SAR1B antibody, anti SARA2 antibody, anti SAR
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 51128

orb19583 (0.03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing SAR1B (RC210593) and probed with orb19583 (mock transfection in first lane), tested by Origene.