SAR1 Antibody

• Catalog Number: orb1246655
• Category: Antibodies
• Description: SAR1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-HSRLVESKVELNA
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SAR1A
• UniProt ID: Q9NR31
• NCBI: NP_064535.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SAR1A, SAR1 gene homolog A (S. cerevisiae), SAR1,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 23 kDa band observed in lysates of NIH3T3 (calculated MW of 22.4 kDa according to Human NP_064535.1 and to Mouse NP_033146.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin and Small Intestine. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246655 (0.1 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246655 (3.75 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.