SAE2 Antibody

SKU: orb1246990

Description

SAE2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:This product has been successfully used in Western blot on Mouse: Kanemaru A, Saitoh H., Biosci Biotechnol Biochem. 2013;77(7):1575-8. PMID: 23832333.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: ALSRGLPRELAEA-C
• Target: SAE2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UBA2, SAE2, SUMO-1 activating enzyme subunit 2

orb1246990 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining.

orb1246990 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and weak cytoplasmic weak staining.

Quick Database Links

Gene Symbol

SAE2

UniProt

Q9UBT2

RefSeq (Protein):NP_005490