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SAE2 Antibody

SKU: orb1246990

Description

SAE2 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IF, WB
ReactivityHuman, Mouse
Predicted ReactivityBovine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:This product has been successfully used in Western blot on Mouse: Kanemaru A, Saitoh H., Biosci Biotechnol Biochem. 2013;77(7):1575-8. PMID: 23832333.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: 10µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: ALSRGLPRELAEA-C
TargetSAE2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

UBA2, SAE2, SUMO-1 activating enzyme subunit 2

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
ProteinNP_005490

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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