SAE2/UBA2 Antibody

• Catalog Number: orb18565
• Category: Antibodies
• Description: Goat polyclonal to SAE2
• Target: SAE2 / UBA2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ALSRGLPRELAEA
• RRID: AB_10748171
• MW: 74
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:16000, IHC-P: 10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments showed an approx. 35kDa band in Human Testis and Brain extracts at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 74KDa according to NP_005490. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti UBA2 antibody, anti SAE2 antibody, anti SUMO-
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 10054

Immunofluorescence analysis of U2OS cells using SAE2 antibody

Immunofluorescence analysis of HeLa cells using SAE2 antibody