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Catalog Number | orb1246991 |
---|---|
Category | Antibodies |
Description | SAE1 Antibody |
Target | SAE1 |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Bovine, Canine |
Form/Appearance | Liquid |
Concentration | 500 ug/mL |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Immunogen | The immunogen for this antibody is: MKGNGIVECLGPK |
UniProt ID | Q9UBE0 |
Tested applications | ELISA, WB |
Application notes | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in Jurkat and 293 cell lysates (predicted MW of 40kDa according to NP_005491). Recommended for use at 1ug/ml. |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | SAE1, AOS1, SUA1, HSPC140, SUMO-1 activating enzym Read more... |
Research Area | Cancer Biology |
Note | For research use only |
NCBI | NP_005491.1 |
Expiration Date | 12 months from date of receipt. |
orb1246991 staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ELISA, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |