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SAE1 Antibody

Catalog Number: orb1246991

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SizePriceQuantity
0.1 mg$ 880.00
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DispatchUsually dispatched within 5-10 working days
Catalog Numberorb1246991
CategoryAntibodies
DescriptionSAE1 Antibody
TargetSAE1
ClonalityPolyclonal
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityBovine, Canine
Form/AppearanceLiquid
Concentration500 ug/mL
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ImmunogenThe immunogen for this antibody is: MKGNGIVECLGPK
UniProt IDQ9UBE0
Tested applicationsELISA, WB
Application notesPeptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in Jurkat and 293 cell lysates (predicted MW of 40kDa according to NP_005491). Recommended for use at 1ug/ml.
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesSAE1, AOS1, SUA1, HSPC140, SUMO-1 activating enzym
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Research AreaCancer Biology
NoteFor research use only
NCBINP_005491.1
Expiration Date12 months from date of receipt.
SAE1 Antibody

orb1246991 staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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