Cart summary

You have no items in your shopping cart.

Goat anti-SAE1 / AOS1 Antibody

Catalog Number: orb18563

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb18563
CategoryAntibodies
DescriptionGoat polyclonal antibody to SAE1
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, IF
ReactivityBovine, Canine, Human
Dilution rangeELISA: 1:8000, WB: 1 μg/ml
ConjugationUnconjugated
MW38.4
TargetSAE1 / AOS1
Entrez10055
Protein SequenceMKGNGIVECLGPK
RRIDAB_10755561
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti SAE1 antibody, anti AOS1 antibody, anti SUA1
Read more...
NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 40kDa band observed in Jurkat and 293 cell lysates (predicted MW of 40kDa according to NP_005491). Recommended for use at 1 μg/ml.
Expiration Date12 months from date of receipt.
Goat anti-SAE1 / AOS1 Antibody

orb18563 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Goat anti-SAE1 / AOS1 Antibody

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Goat anti-SAE1 / AOS1 Antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Goat anti-SAE1 / AOS1 Antibody

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.