S100A9 Antibody / MRP14 / Calgranulin B / Calprotectin L1H

• Catalog Number: orb750149
• Category: Antibodies
• Description: S100A9, also known as Calgranulin B or MRP14 (and Calprotectin L1H), is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgM, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human protein was used as the immunogen for the S100A9 antibody.
• UniProt ID: P06702
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
• Application notes: Titering of the S100A9 antibody may be required for optimal performance.1. Not suitable for frozen tissue testing.
• Antibody Type: Primary Antibody
• Clone Number: PS1A9-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This S100A9 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.