Goat anti-S100A9 Antibody

SKU: orb20117

Description

Goat polyclonal antibody to S100A9

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.5-2 μg/ml, IHC-P: 2.5ug/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Lung shows nuclear staining in select cells, Recommended concentration, 2-4ug/ml.WB: Approx 16kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 13.2kDa according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15kDa) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16;280(50):41521-9.; PMID: 16216873). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: S100A9
• Molecular Weight: 13.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti S100A9 antibody, anti S100 calcium binding protein A9 antibody, anti 60B8AG antibody, anti CAGB antibody, anti CFAG antibody, anti CGLB antibody, anti L1AG antibody, anti LIAG antibody, anti MAC387 antibody, anti MRP14 antibody, anti NIF antibody, anti P14 antibody, anti S100 calcium binding protein A9 (calgranulin B) antibody, anti S100 calcium-binding protein A9 antibody, anti S100 calcium-binding protein A9 (calgranulin B) antibody, anti calgranulin B antibody

1 µg/mL staining of Human Bone Marrow (A) and (0.5 µg/mL) Gastrointestinal cancer (B) lysate and negative control HepG2 (C) lysate. 5 µg protein in RIPA buffer). Detected by chemiluminescence.

2.5 µg/mL staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

7 µg/mL staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Quick Database Links

Gene Symbol

S100A9

NCBI Gene ID

6280