S100A9 Antibody / MRP14 / Calgranulin B / Calprotectin L1H

• Catalog Number: orb749776
• Category: Antibodies
• Description: Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP14); expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S100A9/1011
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgM, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the S100A9 antibody.
• Dilution range: Immunohistochemistry (FFPE; not suitable for frozen tissues): 1-2ug/ml for 30 min at RT
• Purity: PEG precipitation
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This S100A9 antibody is available for research use only.
• UniProt ID: P06702
• Storage: Store the S100A9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the S100A9 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
• Expiration Date: 12 months from date of receipt.