S100A8 Antibody

SKU: orb1259567

Description

S100A8 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human S100A8 (NP_002955.2).
• Target: S100A8
• Molecular Weight: Observed: 11kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

60B8AG, CAGA, calgranulin A, calgranulin-A, calprotectin L1L subunit, CFAG, CGLA, CP-10, cystic fibrosis antigen, L1Ag, leukocyte L1 complex light chain, MA387, MIF, migration inhibitory factor-related protein 8, MRP8, MRP-8, NIF, OTTHUMP00000015329, P8, protein S100-A8, S100 calcium binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, urinary stone protein band A

Western blot analysis of extracts of various cell lines, using S100A8 antibody (orb1259567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human tonsil using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human skin using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of human skin cancer using S100A8 Polyclonal Antibody (orb1259567) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

S100A8

UniProt

P05109