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S100A8 Antibody
SKU: orb1259567
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human S100A8 (NP_002955.2). |
| Target | S100A8 |
| Molecular Weight | Observed: 11kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−60B8AG, CAGA, calgranulin A, calgranulin-A, calprotectin L1L subunit, CFAG, CGLA, CP-10, cystic fibrosis antigen, L1Ag, leukocyte L1 complex light chain, MA387, MIF, migration inhibitory factor-related protein 8, MRP8, MRP-8, NIF, OTTHUMP00000015329, P8, protein S100-A8, S100 calcium binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, urinary stone protein band A
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Western blot analysis of extracts of various cell lines, using S100A8 antibody (orb1259567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human skin using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of human skin cancer using S100A8 Polyclonal Antibody (orb1259567) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
S100A8
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
S100A8 Antibody (orb1259567)
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